Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Bottom-up single-molecule strategy for understanding subunit function of tetrameric β-galactosidase
doi: 10.1073/pnas.1805690115
Figure Lengend Snippet: Statistical analysis of β-gal populations. (A) Histogram of β-gal activities. T1∼T4 labels indicate the number of active monomers in the expressed β-gal. Dashed lines with different colors represent multipeak Gaussian fitting using OriginPro software (Origin Lab): T1, black; T2, red; T3, blue; and T4, pink. Turnover rates of enzymes are calculated by comparing the raw intensity changes with a resorufin calibration curve (SI Appendix, Fig. S8). (B) Peak positions. The peak positions are acquired through Gaussian fitting of the activity histogram in A.
Article Snippet: Dashed lines with different colors represent multipeak Gaussian fitting using OriginPro software (Origin Lab): T1, black; T2, red; T3, blue; and T4, pink.
Techniques: Software, Activity Assay